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TA Cloning
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We have exploited the technique of TA cloning of a gene responsible for antibiotic resistance i.e Kanamycin resistance by following the basic steps involved in the cloning process.Background of the invention An essential requirement for effective genetic engineering of bacteria and other cells propagated in cell cultures is the capacity to select the cells with a specific genotypic alteration. The most common selection strategy in recombinant DNA technology is to include a selection marker in the cloning vector or plasmid. A selection marker can be a cloned gene or a DNA sequence, which allows the separation of the host cells containing the selection marker from those not containing it. The selection marker together with a suitable selection medium maintains the cloning vector in the cells. Otherwise, since the replication of plasmids is an energetic burden for the bacterial host, in a growing cultures the bacteria, which have lost the plasmid, would have a growth advantage over the cells with the plasmid.
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