Enzyme Microfluidics

Microfluidic Enzymology, Volume 737 in the Methods in Enzymology series, presents detailed methodological descriptions and protocols covering a wide range of microfluidics-based approaches for mechanistic enzymology, high-throughput screening, and enzyme engineering campaigns. The volume includes both established methods as well as recent advances in the field. Chapters include protocols for fabricating and running microfluidic devices, state-of-the-art high-throughput droplet and spatial array-based methods, new methods for activity detection, and recent developments integrating microfluidic methods with structural biology and machine learning approaches.

• Presents detailed protocols for a range of state-of-the-art microfluidic approaches for enzymology, helping make these accessible to non-specialist labs.
• Presents recent advances in high-throughput enzyme activity detection including fluorescence, absorbance, mass spectrometry, and droplet physical properties.
• Presents both basic protocols for establishing microfluidic workflows as well as cutting edge technologies for accelerating characterization and discovery of enzymes.